Rapid methods in bacterial DNA fingerprinting

DNA fingerprinting of crude bacterial lysates using degenerate RAPD primers.

Methods for identifying isolates of various pathogenic bacteria by DNA fingerprinting with random primers (RAPD) have been described recently. In these methods many primers are screened and the primers that generate the most informative DNA pattern are selected. A new strategy that simplifies the primer selection process for RAPD fingerprinting has been developed in our laboratory. In this appr...

Rapid DNA extraction of bacterial genome of Staphylococcus aureus using laundry detergents and assessment of the efficiency of DNA in downstream process using PCR

Abstract Background and objectives: Genomic DNA extraction of bacterial cells is of processes performed normally in most biological laboratories therefore, various methods have been offered, manually and kit, which may be time consuming and costly. In this paper, genomic DNA extraction of Staphylococcus aureus was investigated using some laundry detergent brands available in Iran to achieve ...

Rapid Methods of Staining Bacterial Spores at Room Temperature.

Lechtman, M. D. (University of Southern California, Los Angeles), J. W. Bartholomew, A. Phillips, and M. Russo. Rapid methods of staining bacterial spores at room temperature. J. Bacteriol. 89:848-854. 1965.-Spores of Bacillus subtilis var. niger were stained in 2 min at room temperature, after suitable pretreatment, with a dye reagent composed of 2% crystal violet in 1% phenol and 26% ethanol....

A DNA Fingerprinting Exercise for

"Hands on" laboratory exercises are excellent teaching tools because they have the potential to reach students with all types of learning styles. Unfortunately the large monetary investment required to obtain supplies and equipment and train instructors prevents many schools from providing hands-on learning experiences with DNA technology. Students in non-laboratory courses are even less likely...

DNA Fingerprinting and RFLP Analysis